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RT-PCR was performed using the SsoFast EvaGreen Supermix, in a CFX96 real time-PCR system (Bio-Rad, Hercules, CA, USA), with the primers defined in Supplemental Table 2, at 500 nM

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Size:10mg-Reconstituted

It keeps the engine running smoothly and ensures the body of the car (the gut lining and muscles) remains intact